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3 plasmids (pGL1124, pGL1224 and pGL1217) had been made to enable the replacement of 1 allele of CYC9Lister 427 cells. Trypanosomes were transfected with Every plasmid independently, and then subjected to your next transfection with one of the other plasmids, with a distinct resistance gene, with all pairwise combinations done.RNAi. Cells ended up